Part:BBa_K5210017
Pompc-LuxCDEFG
Pompc comes from E.coli and is involved in osmotic regulation. In case of high osmolarity in the medium, the protein kinase EnvZ, located in the cytoplasmic membrane, phosphorylates the transcription factor OmpR. Phosphorylated OmpR binds the ompC promoter and activates the transcription of dowmstream LuxCDEFG.
Usage and Biology
We conducted a literature review and found that bacteria can sense changes in external osmotic pressure via the EnvZ/OmpR two-component system [1]. The EnvZ/OmpR system is prevalent in Gram negative bacillus, and EnvZ regulates OmpR by sensing changes in osmotic pressure, which subsequently affects the expression levels of membrane pore proteins OmpC and OmpF expression levels. By constructing gene circuits, we could achieve to make the engineered bacteria glow in a high osmotic pressure environment i.e., they start to glow after falling into water.
Reference
[1]Kenney, L. J. & Anand, G. S., EnvZ/OmpR Two-Component Signaling: an Archetype System That Can Function Noncanonically. ecoSal Plus 9 (2020).
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Illegal XbaI site found at 146
Illegal PstI site found at 1494
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Illegal XbaI site found at 146
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